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Pengaruh Rasio Doksorubisin:Apoferritin terhadap Kapasitas dan Efisiensi Enkapsulasi Doksorubisin dalam Apoferritin

Novita, Wiwoho and Maria, Christina P and Mujamilah, Mujamilah and Grace, Tj. Sulungbudi (2017) Pengaruh Rasio Doksorubisin:Apoferritin terhadap Kapasitas dan Efisiensi Enkapsulasi Doksorubisin dalam Apoferritin. Jurnal Rekayasa Proses, 11 (1). pp. 24-29. ISSN 2549-1490

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Abstract

Doxorubicin is a chemotherapy drug which is very toxic and causes many side effects. To reduce side effects, doxorubicin can be encapsulated by apoferritin into apoferritin-doxorubicin (Apo-Dox) system. In this research, various mass of doxorubicin i.e. 0.17 mg (S1), 0.26 mg (S2), 0.35 mg (S3), and 0.52 mg (S4) were encapsulated with 21.50 mg apoferritin. Encapsulation process was carried out by lowering pH medium for apoferritin dis-assembly, doxorubicin addition and dialysis for gradual and controlled pH-increase of medium to support re-assembly of apoferritin and doxorubicin encapsulation. End-result samples were then centrifuged and washed to separate the unreacted oxorubicin and apoferritin’s subunits. Doxorubicin encapsulation efficiency was determined using microplate reader spectrophotometry. The highest encapsulation capasity was 3.87 g dox/mg apo for S4 samples. Increasing the weight of doxorubicin gives more significant effect on increasing the reactive weight of apoferritin, which reached 93.73% (S4 sample). Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis confirm that apoferritin is in the Apo-Dox sample.

Item Type: Article
Subjects: Daur Bahan Bakar Nuklir dan Bahan Maju > Bahan Struktur dan Bahan Maju > Teknik Sintesis Bahan Maju
Divisions: Pusat Sains dan Teknologi Bahan Maju
Depositing User: EDITOR PSTBM BATAN
Date Deposited: 09 May 2018 02:13
Last Modified: 18 May 2018 05:42
URI: http://repo-nkm.batan.go.id/id/eprint/1177

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