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PRODUCTION OF IMMUNORADIOMETRICASSA Y (IRMA) CA 15.3 KIT COMPONENT FOR DETECTION OF BREAST CANCER

Widayati, Puji and Ariyanto, Agus and Sutari, Sutari and Mondrida, Gina and Darwati, Siti (2008) PRODUCTION OF IMMUNORADIOMETRICASSA Y (IRMA) CA 15.3 KIT COMPONENT FOR DETECTION OF BREAST CANCER. Jurnal Radioisotop dan Radiofarmaka, 11. ISSN 1410-8542

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Abstract

YKI has ranked that breast cancer as a second deseas in causing death of Indonesian women (, 2007). This phenomenon has been caused by the low level of public awareness in early detection of cancers. As a consequences this kind of cancer has generally been diagnosed in advanced stadium which is difficult to be treated In spite of this, the disease actually can be detected early by measuring level of CA 15.3, a tumor marker for breast cancer. One of such in vitro method is immunoradiometricassay (IRMA) for CA 15.3. The CA 15.3 itself is a glycoprotein of heterogen compound capable of reacting with monoclonal antibody CA 15.3. Production of the IRMA CA 15.3 kit has been performed in the Center for Radioisotope and Radiopharmac.eutical, National Nuclear Energy Agency of Indonesia. Optimization of the kit component has been carried out using several parameter including type of monoclonal antibody for tracer and buffer coating type. The results showed that monoclonal anti CA M37901M type is better than M37552M type for tracer production. The M3790lM gave yield about 79.51%, with specific activity 29.12 ~Ci/~g, radiochemical purity 94.21% and %B/T about 11.94%. Several buffers have been evaluated and 0.05 M pH 9.6 carbonate bicarbonate buffer showed the highest specific binding when it was used as coating buffer. Preparation of IRMA CA 15.3 standard solution gave a linier relation between CA 15.3 concentration and the maximum binding (%B/T) Y=0.227X+0.5177 and correlation coefficient R 0.9840

Item Type: Article
Subjects: Isotop dan Radiasi > Produksi Isotop dan Sumber Radiasi > Teknik Produksi Radiofarmaka
Divisions: Pusat Teknologi Radioisotop dan Radiofarmaka
Depositing User: USER PTRR BATAN
Date Deposited: 15 Jul 2018 14:14
Last Modified: 15 Jul 2018 14:14
URI: http://repo-nkm.batan.go.id/id/eprint/3435

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