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Cytogenetic and Molecular Damages in Blood Lymphocyte of Inhabitants Living in High Level Natural Radiation Area (HLNRA) of Botteng Village, Mamuju, West Sulawesi, Indonesia

Mukh, Syaifudin and Sofiati, Purnami and Tur, Rahardjo and Iin, Kurnia and Nastiti, Rahajeng and Darlina, Darlina and Siti, Nurhayati and Dwi, Ramadhani and Eko, Pudjadi (2018) Cytogenetic and Molecular Damages in Blood Lymphocyte of Inhabitants Living in High Level Natural Radiation Area (HLNRA) of Botteng Village, Mamuju, West Sulawesi, Indonesia. Radiation Environment Medicine, 7 (2). pp. 65-76.

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Abstract

An evaluation on the cytogenetic and DNA damages caused by natural radiation was done in eighty eight person living in Botteng village as HLNRA and their matched control in Keang village as normal level natural radiation areas (NLNRA). Their bloods were collected with their full informed consent and culture set up for cytogenetic evaluation as well as nucleic acid damage observation with comet assay and γ -H2AX that were done according to the standard procedures. Lymphocytes were scored manually under microscopic observation for the presence of chromosome aberrations. Radiosensitivity of cells evaluation was also done by challenging these bloods to 1.5 Gy of gamma rays before being cultured. The evaluation showed that frequency of chromosome aberration in HLNRA group was lower (0.00081) compared to control group (0.00125). However, frequency of micronucleus (MN) in HLNRA group was higher (0.0204) than that in control area (0.0172). Nucleoplasmic bridge and nuclear budding are found in extremely low frequency. DNA damage observation with comet assay showed a difference between study and control group, however γ-H2AX analysis showed no any effects observed in lymphocytes for both groups. Our data did not reveal any significant difference (p>0.05) in HLNRA as compared to NLNRA in all parameters (cytogenetics biomarkers) studied which is supported by the nucleic acid damage evaluation. There was a less radiosensitivity observed in lymphocytes of HLNRA group in comparison with controls

Item Type: Article
Subjects: Keselamatan dan Keamanan Nuklir > Keselamatan Lingkungan > Keselamatan Radiasi Lingkungan
Divisions: Pusat Teknologi Keselamatan dan Metrologi Radiasi
Depositing User: USER PTKMR BATAN
Date Deposited: 01 Sep 2018 14:11
Last Modified: 01 Sep 2018 14:11
URI: http://repo-nkm.batan.go.id/id/eprint/3819

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