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Polymorphism of XRCC1 Gene Exon 6 (Arg194Trp) in Relation to Micronucleus Frequencies in Hospital Radiation Workers

Harry Nugroho Eko, Surniyantoro and Yanti, Lusiyanti and Tur, Rahardjo and Devita, Tetriana and Siti, Nurhayati and Hiroyuki, Date (2018) Polymorphism of XRCC1 Gene Exon 6 (Arg194Trp) in Relation to Micronucleus Frequencies in Hospital Radiation Workers. Atom Indonesia, 44 (2). pp. 105-111. ISSN 2356-5322

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Abstract

The genetic polymorphism of deoxyribonucleic acid (DNA) repair genes plays important roles in regulating individual sensitivity to ionizing radiation, maintaining DNA integrity, and preventing cancer and DNA damage. X-ray repair crosscomplementary group 1 (XRCC1) as one of the members of base excision repair (BER) is involved in the repairement of oxidized bases and single-strand breaks DNA after exposure by reactive oxygen species (ROS), including ionizing radiation. This study aimed to examine the correlation between XRCC1 exon 6 gene polymorphism and micronucleus (MN) frequency in radiation workers and their relation to age, gender, smoking status and years of exposure. This study involved 81 hospital radiation workers and 20 controls from several hospitals in Indonesia. Genotyping of XRCC1 exon 6 gene polymorphism and MN assay were performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and cytokinesis-block micronucleus assay (CBMN assay), respectively. The results indicated that MN frequency was significantly higher in the exposed workers than in the controls (15.38 ± 7.72 versus 9 ± 5.49; p = 0.001). Radiation workers with heterozygous alleles for XRCC1 polymorphisms showed a significantly higher MN frequency than controls with the same genotypes (17.5 ± 8.36 versus 7.44 ± 5.05; p = 0.002). The confounding factors, like gender and age, were significantly associated with increased MN frequency both in radiation workers and controls. Smoking status was significantly associated with MN frequency in the controls only, while years of exposure did not affect MN frequency either in radiation workers or controls. These results suggest that the genetic polymorphism of XRCC1 gene exon 6 with a mutant heterozygous/cytosine-thymine (CT) variant demonstrated an association with the extent of DNA damage in the hospital radiation workers in this study. In the subsequent studies, it will be necessary to examine the DNA repair genes polymorphism in populations with controlled non-genetic factors, such as lifestyle, environment, and exercise, that affect the MN frequency as a biomarker of DNA damage

Item Type: Article
Subjects: Keselamatan dan Keamanan Nuklir > Keselamatan Radiasi dan Kesehatan kerja
Divisions: Pusat Teknologi Keselamatan dan Metrologi Radiasi
Depositing User: USER PTKMR BATAN
Date Deposited: 01 Sep 2018 14:14
Last Modified: 01 Sep 2018 14:14
URI: http://repo-nkm.batan.go.id/id/eprint/3823

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