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Penggunaan metode radioassay teknik fase padat dalam reaksi fiksasi a--Kobratoksin terhadap reseptor koligemik

Z, Nurlaila (2000) Penggunaan metode radioassay teknik fase padat dalam reaksi fiksasi a--Kobratoksin terhadap reseptor koligemik. Penggunaan metode radioassay teknik fase padat dalam reaksi fiksasi a--Kobratoksin terhadap reseptor koligemik. pp. 1-5.

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Abstract

USING OF SOLID PHASE RADIOASSAY MEnlOD IN THE FIXAllON REACTION OF aCOBRA TOXIN TO CHOliNERGIC RESEPTOR. a-cobratoxin is a protein tl1at is in the snake venom of Naja naja kaouthia. If, it enter into the body, would be fixed specifically to the cholinergic receptor, causing muscle and breathing paralyze. Determination of functional groups in this fixation could be accomplish by filtration radioassay method with labelled radioactive a-cobratoxin. Beside that, the functional groups in this fixation could be determined by solidPl1ase raaJoassay method. The determination of optimum condition of acobratoxin fixation to cholinergic receptor with labeled tritium a-cobratoxin (3H-a-cobratoxin) using this method has been carried out. Optimum condition of fixation was reached at 20 J:tg of cholinergic receptor coated on the ELISA plat with ~-a-cobratoxin activity range 5.0 104 to 7.5 104 cpm and 4 hours of minimum time incubation at room temperature. Competitive reaction betweell ~-a-cobratoxin and a-cobratoxin to the cholinergic receptor gave 6.50 10-9 M of the 50% of inhibition competitive value (ICso) Evaluation of this method using (N.-acetyllysin-23)a-cobratoxin derivative showed tl1athe compound was inhibited in the fixation to cholinergic receptor with IC5o = 9.50 10-9 M. From this experiment was obtained that this method could be used to determine the active group of the toxin which function in the fixation reaction to cholinergic receptor.

Item Type: Article
Subjects: Isotop dan Radiasi > Pemanfaatan Isotop dan Radiasi > Bidang Kesehatan
Divisions: Pusat Sains dan Teknologi Nuklir Terapan
Depositing User: user pstnt batan
Date Deposited: 03 May 2018 07:25
Last Modified: 03 May 2018 07:25
URI: http://repo-nkm.batan.go.id/id/eprint/693

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