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SINTESIS DAN KARAKTERISASI NUKLEOTIDA BERTANDA [α-32P]ATP

Rahman, Wira and Sarmini, Endang and Herlina, Herlina and Abidin, Abidin and Triyanto, Triyanto and Hambali, Hambali and Nurbaiti, Santi (2019) SINTESIS DAN KARAKTERISASI NUKLEOTIDA BERTANDA [α-32P]ATP. Jurnal Riset Kimia, 10 (1). pp. 30-35. ISSN 2476-8960

[img] Text (The utilization of nuclear technology in health sector with molecular techniques is increasingly developed today, especially in Indonesia. One of which is nucleotide compound marked with [α-32P]ATP, this compound has been used as tracer for deoxyribonucl)
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Abstract

The utilization of nuclear technology in health sector with molecular techniques is increasingly developed today, especially in Indonesia. One of which is nucleotide compound marked with [α-32P]ATP, this compound has been used as tracer for deoxyribonucleic acid (DNA)/ ribonucleic acid (RNA) in the study of various physiological and pathological processes. [α-32P]ATP is synthesized through several stages of continuous reaction in one reaction vessel. It begins with synthesis of [γ-32P]ATP through an enzymatic reaction, using H332PO4 and ADP, and enzymes of lactate dehydrogenase, 3-phosphoglycerate phosphokinase and glyceraldehide 3-phospho dehydrogenase; followed by phosphorilation of 3’AMP with T4 polinucleotide kinase enzyme to produce 3’-[5’-32P]ATP. The result is hydrolyzed with nuclease P1 enzyme to produce [5’-32P]AMP. The unreacted [γ-32P] is degraded by the addition of hexokinase enzyme and glucose. At the final stage of the reaction, the [5’-32P]AMP is phosphorilated using phosphoenol-piruvat, piruvat kinase, and myokinase to produce [α-32P]ATP. The test results show that the every stage of reaction is characterized using TLC method, PEI cellulose paper as stationary phase and KH3PO4 0,5 M pH 3,5 as mobile phase. At the end of reaction, the yield of [α-32P]ATP reaches 71,7%, at Rf = 0,2.

Item Type: Article
Subjects: Isotop dan Radiasi > Produksi Isotop dan Sumber Radiasi > Teknik Produksi Radioisotop
Divisions: Pusat Teknologi Radioisotop dan Radiofarmaka
Depositing User: USER PTRR BATAN
Date Deposited: 18 Oct 2019 16:55
Last Modified: 18 Oct 2019 16:55
URI: http://repo-nkm.batan.go.id/id/eprint/9384

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