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Evaluation of 99mTc-Ethambutol Radiopharmaceutical Using High Performance Liquid Chromatography with Radioactive Detector (Radio-HPLC)

Sriyani, Maula Eka Evaluation of 99mTc-Ethambutol Radiopharmaceutical Using High Performance Liquid Chromatography with Radioactive Detector (Radio-HPLC). Indonesian Journal of Pharmaceutical Science and Technology. (Submitted)


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99mTc-ethambutol is a radiopharmaceuticals used to determine the location of Mycobacterium tuberculosis that causes TB disease. To prepare this radiopharmaceuticals, supporting compounds such as mannitol as filler, SnCl2 as a reducing agent and sodium pyrophosphate as co-ligand are required in addition to ethambutol and radionuclide compounds. The radiopharmaceuticals is made in the form of a lyophilized kit without 99mTc radionuclides. The aim of this study was to determine the effect of reducing agents on 99mTc-ethambutol preparation using the High Performance liquid chromatography (HPLC) equiped with radiodetector (radio-HPLC) to the quality of radiopharmaceuticals. In the previous study, the determination of radiochemical purity only used TLC by determining the amount of impurities TcO2 and TcO4 - . This study was carried out more profoundly by using radio-HPLC to ensure the quality of radiopharmaceutical products. The test were performed using paper chromatography, electrophoresis, radio-HPLC, and stability test of 99mTc-ethambutol radiopharmaceuticals. The results showed that the radiopharmaceutical kit of ethambutol is performing good physical characterictics and high radiochemical purity of 99mTc-ethambutol in analysis using radio-HPLC as well as paper chromatography and electrophoresis. The retention time (tr ) of 99mTc-ethambutol were found at 7.97 minutes using UV and radioactive detector, C18 column, isocratic system using phosphate buffer 0.2 M pH 7.4 and Acetonitrile (90 :10), flow rate 0.8 mL/min. The radiochemical purity of 99mTc-ethambutol is still remain high (91.44 ± 0.92%) after 27 days of kit were made. From the results, it was concluded that radio-HPLC analysis provides better information on radiochemical purity as well as chemical purity of the labeling of 99mTc-ethambutol. Keywords: 99mTc-ethambutol, Radio-HPLC, TB disease, Mycobacterium tuberculosis, Radiopharmaceuticals

Item Type: Article
Subjects: Isotop dan Radiasi > Pemanfaatan Isotop dan Radiasi > Bidang Kesehatan
Divisions: Pusat Sains dan Teknologi Nuklir Terapan
Depositing User: user pstnt batan
Date Deposited: 30 Mar 2021 03:22
Last Modified: 30 Mar 2021 03:22
URI: http://repo-nkm.batan.go.id/id/eprint/9508

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